rabbit polyclonal anti nox4 antibody  (Novus Biologicals)

Journal: PLOS ONE

Article Title: Trolox and recombinant Irisin as a potential strategy to prevent neuronal damage induced by random positioning machine exposure in differentiated HT22 cells

doi: 10.1371/journal.pone.0300888

Figure Lengend Snippet: (a–c) Western blotting analysis for Akt and B-cell lymphoma 2 (Bcl-2): RPM exposure significantly reduced Akt and Bcl-2 expressions, while treated cells showed similar or higher levels of these proteins compared with the normogravity regime. (d) Intracellular levels of reactive oxygen species (ROS) measurement ( n = 15 from N = 5 experiments): RPM exposure promoted a significant increase in oxidative stress (****p<0.0001), which was completely counteracted by combined treatment; whereas treatment with Trolox or r-Irisin only partially reduced the oxidative stress induced by RPM exposure. Data were expressed as mean ± standard deviation and were compared by one-way ANOVA and Tukey’s multiple comparison test. (e–i) Immunofluorescence analysis for NADPH oxidase 4 (NOX4): Nuclei are stained with DAPI (blue) in HT22 cells, while NOX4 immunostaining is depicted in red (arrows) in HT22 cells. 40× images, scale bar represents 100 μm.

Article Snippet: In detail, after fixation in 4% paraformaldehyde dissolved in 0.9% saline solution for 30 min, cell cultures were pretreated with ethylenediaminetetraacetic acid (EDTA) citrate, pH 7.8 for 20 min at 95°C, and incubated for 1 h with mouse monoclonal anti-NMDAR1 antibody (556308, BD PharmingenTM, United Sates), rabbit polyclonal anti-MAP2 antibody (ab32454, AbCam, Cambridge, United Kingdom), mouse monoclonal anti-ChAT antibody (CL3173, Novus Biologicals, Littleton, CO, United Sates), or rabbit polyclonal anti-NOX4 antibody (NB110-58849, Novus Biologicals, Littleton, CO, United Sates).

Techniques: Western Blot, Standard Deviation, Comparison, Immunofluorescence, Staining, Immunostaining

Journal: Antioxidants (Basel, Switzerland)

Article Title: Corynoxeine Supplementation Ameliorates Colistin-Induced Kidney Oxidative Stress and Inflammation in Mice.

doi: 10.3390/antiox14050593

Figure Lengend Snippet: Figure 6. The expression changes of TGF-β and NOX4 proteins in the kidneys of mice. (A) Represen- tative image of immunohistochemical staining. (B) The quantitative analysis of immunohistochemical staining. Data were shown as mean ± S.D. (n = 5). ** p < 0.01, compared to the untreated control. ## p < 0.01, compared to the colistin-only treatment group. Bar = 50 µm.

Article Snippet: Rabbit polyclonal antibodies targeting NOX4 and TGF-β (both diluted at 1:200; ProteinTech Group, Inc., Chicago, IL, USA) were utilized.

Techniques: Expressing, Immunohistochemical staining, Staining, Control

Journal: Journal of Cancer

Article Title: LncRNA C2orf27A Promotes Gastric Cancer by Sponging MiR-610 and Elevating NOX4 Expression

doi: 10.7150/jca.100621

Figure Lengend Snippet: Figure 6. MiR-610 blocks the malignant behavior of GC cells by inhibiting NOX4 expression. (A, B) The data from TCGA-STAD and GTEx indicated that NOX4 expression was significantly higher in GC tissues than that in normal gastric tissues (unpaired and paired samples). (C) The correlation analysis of miR-610 and NOX4 expression in GC tissues based on TCGA-STAD. (D) Bioinformatics analysis using DIANA-microT-CDS showed the putative binding site between miR-610 and NOX4. (E) Luciferase reporter gene assays revealed that miR-610 negatively regulated the luciferase activity of NOX4-WT rather than that of the mutant NOX4-MUT. (F, G) qRT-PCR and western blot were used to detect NOX4 levels after transfecting miR-610 mimics into HGC-27 cells. (H-K) NOX4 overexpression partially offset the functions of miR-610 mimics on proliferation, colony formation, cell cycle progression, and apoptosis of HGC-27 cells. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, and ***P <0.001, ns: no significance.

Article Snippet: After blocking with 10% skim milk for an hour at room temperature, the membranes were incubated overnight at 4°C with rabbit polyclonal antibodies against NOX4 (1:2000) and GAPDH (1:6000, Proteintech, China).

Techniques: Expressing, Binding Assay, Luciferase, Activity Assay, Mutagenesis, Quantitative RT-PCR, Western Blot, Over Expression

Journal: Journal of Cancer

Article Title: LncRNA C2orf27A Promotes Gastric Cancer by Sponging MiR-610 and Elevating NOX4 Expression

doi: 10.7150/jca.100621

Figure Lengend Snippet: Figure 7. C2orf27A knockdown exerts antitumor effects in GC cells by downregulating NOX4 expression. (A) Correlation analysis of C2orf27A and NOX4 expression in GC cells based on TCGA-STAD. (B, C) qRT-PCR and western blot detected the levels of NOX4 in HGC-27 and NCI-C87 cells after C2orf27A knockdown. (D-G) NOX4 overexpression partially offset the effects of C2orf27A knockdown on proliferation, colony formation, cell cycle progression and apoptosis of HGC-27 cells. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, and **P < 0.01, ***P <0.001, ns: no significance.

Article Snippet: After blocking with 10% skim milk for an hour at room temperature, the membranes were incubated overnight at 4°C with rabbit polyclonal antibodies against NOX4 (1:2000) and GAPDH (1:6000, Proteintech, China).

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Over Expression

Journal: EMBO Reports

Article Title: Eicosapentaenoic acid induces macrophage Mox polarization to prevent diabetic cardiomyopathy

doi: 10.1038/s44319-024-00271-x

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit polyclonal anti-NOX4 WB (1:1000) , Proteintech , Cat# 14347-1-AP RRID: AB_10638146.

Techniques: Sequencing, Staining, Transfection, SYBR Green Assay, Software, TUNEL Assay, Apoptosis Assay, Modification, Cell Isolation, Bicinchoninic Acid Protein Assay